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angiosys 1.0  (TCS Cellworks)

 
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    TCS Cellworks angiosys 1.0
    Influence of saccharide mimetics on endothelial networking (matrigel-assay) (A) and tube formation (B,C). A: The matrigel-assay is used to assess the initial stages of tube formation, i.e., the lining-up and subsequent formation of networks between endothelial cells. In the experiment shown, cells were stimulated with TNF or left untreated for 24 h, then (4-{[(β-D-galactopyranosyl)oxy]methyl}furan-3-yl)methyl hydrogen sulfate (GSF) was added in the concentrations indicated. The cells were then seeded onto the matrigel, which consists of a solubilized complex basement membrane of the EHS mouse tumor , and incubated overnight. B: The tubing assay measures the formation of endothelial tubules in a coculture of HUVEC and fibroblast cells (dots seen in the background). VEGF, which is necessary for proper in vitro tubule formation, was added at day 0 to the cells together with GSF or 3,4-bis{[(β-D-galactopyranosyl)oxy]methyl}furan (BGF) in the concentrations indicated. As one control, tubule formation is shown with VEGF only (untreated) or with 3,4-bis(hydroxymethyl)furan (5 mM control*). The growth media including growth factors and the respective mimetics were changed at days 4 and 7. At day 9 the medium was removed and the endothelial cells were stained with a monoclonal antibody against the cell surface antigen CD31 to allow evaluation of tubule formation. The assay was performed in three independent experiments with the same results in terms of the GSF effect. C: The tubule formation as observed under the microscopic was quantitatively analyzed using Angiosys 1.0 software, which counts the total tubule length, number of tubules and junctions between different tubules. Here, we show the results for total tubule length of an experiment performed as shown in panel B. The values are means and standard deviations of three experiments, * = p < 0.05, *** = p < 0.001 of the GSF exposed cells compared to VEGF alone. ANOVA one-way analysis of variance showed the concentration dependence of the GSF effect to be significant with p < 0.0001. The results for the other parameters reflected the same tendency of inhibitory capacity of GSF with increasing concentrations.
    Angiosys 1.0, supplied by TCS Cellworks, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/angiosys+1%2E0/pmc03388867-310-18-20?v=TCS+Cellworks
    Average 90 stars, based on 1 article reviews
    angiosys 1.0 - by Bioz Stars, 2026-07
    90/100 stars

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    1) Product Images from "An easily accessible sulfated saccharide mimetic inhibits in vitro human tumor cell adhesion and angiogenesis of vascular endothelial cells"

    Article Title: An easily accessible sulfated saccharide mimetic inhibits in vitro human tumor cell adhesion and angiogenesis of vascular endothelial cells

    Journal: Beilstein Journal of Organic Chemistry

    doi: 10.3762/bjoc.8.89

    Influence of saccharide mimetics on endothelial networking (matrigel-assay) (A) and tube formation (B,C). A: The matrigel-assay is used to assess the initial stages of tube formation, i.e., the lining-up and subsequent formation of networks between endothelial cells. In the experiment shown, cells were stimulated with TNF or left untreated for 24 h, then (4-{[(β-D-galactopyranosyl)oxy]methyl}furan-3-yl)methyl hydrogen sulfate (GSF) was added in the concentrations indicated. The cells were then seeded onto the matrigel, which consists of a solubilized complex basement membrane of the EHS mouse tumor , and incubated overnight. B: The tubing assay measures the formation of endothelial tubules in a coculture of HUVEC and fibroblast cells (dots seen in the background). VEGF, which is necessary for proper in vitro tubule formation, was added at day 0 to the cells together with GSF or 3,4-bis{[(β-D-galactopyranosyl)oxy]methyl}furan (BGF) in the concentrations indicated. As one control, tubule formation is shown with VEGF only (untreated) or with 3,4-bis(hydroxymethyl)furan (5 mM control*). The growth media including growth factors and the respective mimetics were changed at days 4 and 7. At day 9 the medium was removed and the endothelial cells were stained with a monoclonal antibody against the cell surface antigen CD31 to allow evaluation of tubule formation. The assay was performed in three independent experiments with the same results in terms of the GSF effect. C: The tubule formation as observed under the microscopic was quantitatively analyzed using Angiosys 1.0 software, which counts the total tubule length, number of tubules and junctions between different tubules. Here, we show the results for total tubule length of an experiment performed as shown in panel B. The values are means and standard deviations of three experiments, * = p < 0.05, *** = p < 0.001 of the GSF exposed cells compared to VEGF alone. ANOVA one-way analysis of variance showed the concentration dependence of the GSF effect to be significant with p < 0.0001. The results for the other parameters reflected the same tendency of inhibitory capacity of GSF with increasing concentrations.
    Figure Legend Snippet: Influence of saccharide mimetics on endothelial networking (matrigel-assay) (A) and tube formation (B,C). A: The matrigel-assay is used to assess the initial stages of tube formation, i.e., the lining-up and subsequent formation of networks between endothelial cells. In the experiment shown, cells were stimulated with TNF or left untreated for 24 h, then (4-{[(β-D-galactopyranosyl)oxy]methyl}furan-3-yl)methyl hydrogen sulfate (GSF) was added in the concentrations indicated. The cells were then seeded onto the matrigel, which consists of a solubilized complex basement membrane of the EHS mouse tumor , and incubated overnight. B: The tubing assay measures the formation of endothelial tubules in a coculture of HUVEC and fibroblast cells (dots seen in the background). VEGF, which is necessary for proper in vitro tubule formation, was added at day 0 to the cells together with GSF or 3,4-bis{[(β-D-galactopyranosyl)oxy]methyl}furan (BGF) in the concentrations indicated. As one control, tubule formation is shown with VEGF only (untreated) or with 3,4-bis(hydroxymethyl)furan (5 mM control*). The growth media including growth factors and the respective mimetics were changed at days 4 and 7. At day 9 the medium was removed and the endothelial cells were stained with a monoclonal antibody against the cell surface antigen CD31 to allow evaluation of tubule formation. The assay was performed in three independent experiments with the same results in terms of the GSF effect. C: The tubule formation as observed under the microscopic was quantitatively analyzed using Angiosys 1.0 software, which counts the total tubule length, number of tubules and junctions between different tubules. Here, we show the results for total tubule length of an experiment performed as shown in panel B. The values are means and standard deviations of three experiments, * = p < 0.05, *** = p < 0.001 of the GSF exposed cells compared to VEGF alone. ANOVA one-way analysis of variance showed the concentration dependence of the GSF effect to be significant with p < 0.0001. The results for the other parameters reflected the same tendency of inhibitory capacity of GSF with increasing concentrations.

    Techniques Used: Matrigel Assay, Incubation, In Vitro, Staining, Software, Concentration Assay



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    Influence of saccharide mimetics on endothelial networking (matrigel-assay) (A) and tube formation (B,C). A: The matrigel-assay is used to assess the initial stages of tube formation, i.e., the lining-up and subsequent formation of networks between endothelial cells. In the experiment shown, cells were stimulated with TNF or left untreated for 24 h, then (4-{[(β-D-galactopyranosyl)oxy]methyl}furan-3-yl)methyl hydrogen sulfate (GSF) was added in the concentrations indicated. The cells were then seeded onto the matrigel, which consists of a solubilized complex basement membrane of the EHS mouse tumor , and incubated overnight. B: The tubing assay measures the formation of endothelial tubules in a coculture of HUVEC and fibroblast cells (dots seen in the background). VEGF, which is necessary for proper in vitro tubule formation, was added at day 0 to the cells together with GSF or 3,4-bis{[(β-D-galactopyranosyl)oxy]methyl}furan (BGF) in the concentrations indicated. As one control, tubule formation is shown with VEGF only (untreated) or with 3,4-bis(hydroxymethyl)furan (5 mM control*). The growth media including growth factors and the respective mimetics were changed at days 4 and 7. At day 9 the medium was removed and the endothelial cells were stained with a monoclonal antibody against the cell surface antigen CD31 to allow evaluation of tubule formation. The assay was performed in three independent experiments with the same results in terms of the GSF effect. C: The tubule formation as observed under the microscopic was quantitatively analyzed using Angiosys 1.0 software, which counts the total tubule length, number of tubules and junctions between different tubules. Here, we show the results for total tubule length of an experiment performed as shown in panel B. The values are means and standard deviations of three experiments, * = p < 0.05, *** = p < 0.001 of the GSF exposed cells compared to VEGF alone. ANOVA one-way analysis of variance showed the concentration dependence of the GSF effect to be significant with p < 0.0001. The results for the other parameters reflected the same tendency of inhibitory capacity of GSF with increasing concentrations.
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    Influence of saccharide mimetics on endothelial networking (matrigel-assay) (A) and tube formation (B,C). A: The matrigel-assay is used to assess the initial stages of tube formation, i.e., the lining-up and subsequent formation of networks between endothelial cells. In the experiment shown, cells were stimulated with TNF or left untreated for 24 h, then (4-{[(β-D-galactopyranosyl)oxy]methyl}furan-3-yl)methyl hydrogen sulfate (GSF) was added in the concentrations indicated. The cells were then seeded onto the matrigel, which consists of a solubilized complex basement membrane of the EHS mouse tumor , and incubated overnight. B: The tubing assay measures the formation of endothelial tubules in a coculture of HUVEC and fibroblast cells (dots seen in the background). VEGF, which is necessary for proper in vitro tubule formation, was added at day 0 to the cells together with GSF or 3,4-bis{[(β-D-galactopyranosyl)oxy]methyl}furan (BGF) in the concentrations indicated. As one control, tubule formation is shown with VEGF only (untreated) or with 3,4-bis(hydroxymethyl)furan (5 mM control*). The growth media including growth factors and the respective mimetics were changed at days 4 and 7. At day 9 the medium was removed and the endothelial cells were stained with a monoclonal antibody against the cell surface antigen CD31 to allow evaluation of tubule formation. The assay was performed in three independent experiments with the same results in terms of the GSF effect. C: The tubule formation as observed under the microscopic was quantitatively analyzed using Angiosys 1.0 software, which counts the total tubule length, number of tubules and junctions between different tubules. Here, we show the results for total tubule length of an experiment performed as shown in panel B. The values are means and standard deviations of three experiments, * = p < 0.05, *** = p < 0.001 of the GSF exposed cells compared to VEGF alone. ANOVA one-way analysis of variance showed the concentration dependence of the GSF effect to be significant with p < 0.0001. The results for the other parameters reflected the same tendency of inhibitory capacity of GSF with increasing concentrations.
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    Influence of saccharide mimetics on endothelial networking (matrigel-assay) (A) and tube formation (B,C). A: The matrigel-assay is used to assess the initial stages of tube formation, i.e., the lining-up and subsequent formation of networks between endothelial cells. In the experiment shown, cells were stimulated with TNF or left untreated for 24 h, then (4-{[(β-D-galactopyranosyl)oxy]methyl}furan-3-yl)methyl hydrogen sulfate (GSF) was added in the concentrations indicated. The cells were then seeded onto the matrigel, which consists of a solubilized complex basement membrane of the EHS mouse tumor , and incubated overnight. B: The tubing assay measures the formation of endothelial tubules in a coculture of HUVEC and fibroblast cells (dots seen in the background). VEGF, which is necessary for proper in vitro tubule formation, was added at day 0 to the cells together with GSF or 3,4-bis{[(β-D-galactopyranosyl)oxy]methyl}furan (BGF) in the concentrations indicated. As one control, tubule formation is shown with VEGF only (untreated) or with 3,4-bis(hydroxymethyl)furan (5 mM control*). The growth media including growth factors and the respective mimetics were changed at days 4 and 7. At day 9 the medium was removed and the endothelial cells were stained with a monoclonal antibody against the cell surface antigen CD31 to allow evaluation of tubule formation. The assay was performed in three independent experiments with the same results in terms of the GSF effect. C: The tubule formation as observed under the microscopic was quantitatively analyzed using Angiosys 1.0 software, which counts the total tubule length, number of tubules and junctions between different tubules. Here, we show the results for total tubule length of an experiment performed as shown in panel B. The values are means and standard deviations of three experiments, * = p < 0.05, *** = p < 0.001 of the GSF exposed cells compared to VEGF alone. ANOVA one-way analysis of variance showed the concentration dependence of the GSF effect to be significant with p < 0.0001. The results for the other parameters reflected the same tendency of inhibitory capacity of GSF with increasing concentrations.
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    Influence of saccharide mimetics on endothelial networking (matrigel-assay) (A) and tube formation (B,C). A: The matrigel-assay is used to assess the initial stages of tube formation, i.e., the lining-up and subsequent formation of networks between endothelial cells. In the experiment shown, cells were stimulated with TNF or left untreated for 24 h, then (4-{[(β-D-galactopyranosyl)oxy]methyl}furan-3-yl)methyl hydrogen sulfate (GSF) was added in the concentrations indicated. The cells were then seeded onto the matrigel, which consists of a solubilized complex basement membrane of the EHS mouse tumor , and incubated overnight. B: The tubing assay measures the formation of endothelial tubules in a coculture of HUVEC and fibroblast cells (dots seen in the background). VEGF, which is necessary for proper in vitro tubule formation, was added at day 0 to the cells together with GSF or 3,4-bis{[(β-D-galactopyranosyl)oxy]methyl}furan (BGF) in the concentrations indicated. As one control, tubule formation is shown with VEGF only (untreated) or with 3,4-bis(hydroxymethyl)furan (5 mM control*). The growth media including growth factors and the respective mimetics were changed at days 4 and 7. At day 9 the medium was removed and the endothelial cells were stained with a monoclonal antibody against the cell surface antigen CD31 to allow evaluation of tubule formation. The assay was performed in three independent experiments with the same results in terms of the GSF effect. C: The tubule formation as observed under the microscopic was quantitatively analyzed using Angiosys 1.0 software, which counts the total tubule length, number of tubules and junctions between different tubules. Here, we show the results for total tubule length of an experiment performed as shown in panel B. The values are means and standard deviations of three experiments, * = p < 0.05, *** = p < 0.001 of the GSF exposed cells compared to VEGF alone. ANOVA one-way analysis of variance showed the concentration dependence of the GSF effect to be significant with p < 0.0001. The results for the other parameters reflected the same tendency of inhibitory capacity of GSF with increasing concentrations.
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    Influence of saccharide mimetics on endothelial networking (matrigel-assay) (A) and tube formation (B,C). A: The matrigel-assay is used to assess the initial stages of tube formation, i.e., the lining-up and subsequent formation of networks between endothelial cells. In the experiment shown, cells were stimulated with TNF or left untreated for 24 h, then (4-{[(β-D-galactopyranosyl)oxy]methyl}furan-3-yl)methyl hydrogen sulfate (GSF) was added in the concentrations indicated. The cells were then seeded onto the matrigel, which consists of a solubilized complex basement membrane of the EHS mouse tumor , and incubated overnight. B: The tubing assay measures the formation of endothelial tubules in a coculture of HUVEC and fibroblast cells (dots seen in the background). VEGF, which is necessary for proper in vitro tubule formation, was added at day 0 to the cells together with GSF or 3,4-bis{[(β-D-galactopyranosyl)oxy]methyl}furan (BGF) in the concentrations indicated. As one control, tubule formation is shown with VEGF only (untreated) or with 3,4-bis(hydroxymethyl)furan (5 mM control*). The growth media including growth factors and the respective mimetics were changed at days 4 and 7. At day 9 the medium was removed and the endothelial cells were stained with a monoclonal antibody against the cell surface antigen CD31 to allow evaluation of tubule formation. The assay was performed in three independent experiments with the same results in terms of the GSF effect. C: The tubule formation as observed under the microscopic was quantitatively analyzed using Angiosys 1.0 software, which counts the total tubule length, number of tubules and junctions between different tubules. Here, we show the results for total tubule length of an experiment performed as shown in panel B. The values are means and standard deviations of three experiments, * = p < 0.05, *** = p < 0.001 of the GSF exposed cells compared to VEGF alone. ANOVA one-way analysis of variance showed the concentration dependence of the GSF effect to be significant with p < 0.0001. The results for the other parameters reflected the same tendency of inhibitory capacity of GSF with increasing concentrations.

    Journal: Beilstein Journal of Organic Chemistry

    Article Title: An easily accessible sulfated saccharide mimetic inhibits in vitro human tumor cell adhesion and angiogenesis of vascular endothelial cells

    doi: 10.3762/bjoc.8.89

    Figure Lengend Snippet: Influence of saccharide mimetics on endothelial networking (matrigel-assay) (A) and tube formation (B,C). A: The matrigel-assay is used to assess the initial stages of tube formation, i.e., the lining-up and subsequent formation of networks between endothelial cells. In the experiment shown, cells were stimulated with TNF or left untreated for 24 h, then (4-{[(β-D-galactopyranosyl)oxy]methyl}furan-3-yl)methyl hydrogen sulfate (GSF) was added in the concentrations indicated. The cells were then seeded onto the matrigel, which consists of a solubilized complex basement membrane of the EHS mouse tumor , and incubated overnight. B: The tubing assay measures the formation of endothelial tubules in a coculture of HUVEC and fibroblast cells (dots seen in the background). VEGF, which is necessary for proper in vitro tubule formation, was added at day 0 to the cells together with GSF or 3,4-bis{[(β-D-galactopyranosyl)oxy]methyl}furan (BGF) in the concentrations indicated. As one control, tubule formation is shown with VEGF only (untreated) or with 3,4-bis(hydroxymethyl)furan (5 mM control*). The growth media including growth factors and the respective mimetics were changed at days 4 and 7. At day 9 the medium was removed and the endothelial cells were stained with a monoclonal antibody against the cell surface antigen CD31 to allow evaluation of tubule formation. The assay was performed in three independent experiments with the same results in terms of the GSF effect. C: The tubule formation as observed under the microscopic was quantitatively analyzed using Angiosys 1.0 software, which counts the total tubule length, number of tubules and junctions between different tubules. Here, we show the results for total tubule length of an experiment performed as shown in panel B. The values are means and standard deviations of three experiments, * = p < 0.05, *** = p < 0.001 of the GSF exposed cells compared to VEGF alone. ANOVA one-way analysis of variance showed the concentration dependence of the GSF effect to be significant with p < 0.0001. The results for the other parameters reflected the same tendency of inhibitory capacity of GSF with increasing concentrations.

    Article Snippet: Wells were sealed with mounting medium and microscopic quantitative analysis of tube formation was performed with the software Angiosys 1.0, TCS (Cellworks).

    Techniques: Matrigel Assay, Incubation, In Vitro, Staining, Software, Concentration Assay